The plasmid we will be using to clone our gene into is called pLATE 31. It is manufactured as a vector for cloning of genes and expression of proteins from the recombinant DNA.
From this map you can see all of the DNA sequences needed to express a protein from a cloned gene. There is an origin of replication (rep) needed for replication, a T7 promoter (PT7) and T7 terminator (T T7) needed for transcription, and a ribosomal binding sequence (RBS) needed for translation. With all the machinery in place, once our gene is correctly cloned into pLATE31 we will be able to produce a protein product.