So far in this course, you have learned several ways to analyze and purify proteins. Now, it’s time to put these skills together in service of a goal: to express, purify, and determine the turnover rate of the enzyme BglA under standard conditions, and an alternate condition of your own choosing. (Recall the BglA encodes beta-glucosidase; you cloned the BglA gene from Bacillus halodurans into a plasmid, and transformed E. coli with the plasmid.)
There is one new topic and skill for you to learn before the project begins: protein expression. Continue to the next page to learn about it.