Module4 Enzyme Kinetics and Maximal Velocity

The figure below shows a simple mechanism for an enzymatic reaction. In the figure, E stands for enzyme, S for substrate, and P for product. ES is enzyme-substrate complex. Note that there are two steps: the enzyme and substrate must bind each other to form an enzyme-substrate complex before the catalytic step, which produces product, can occur.

In the video, you’ll watch a narrated powerpoint presentation that describes some basic enzyme kinetics experiments.

Enzyme and Substrate can reversibly form an Enzyme-Substrate Complex; this is the Substrate Binding step. Enzyme-Substrate Complex can convert to Enzyme plus Substrate; this is the Catalytic step.
E stands for Enzyme, S for Substrate, P for Product

Watch

PDF version of the slides: Enzyme Kinetics slides.pdf