You should be familiar with most of the equipment you’ll be using to thaw your cells. It’s possible you won’t be familiar with the type of flasks you will be using: a T-flask.

T-Flasks Examples

T-flasks are the standard growth vessel for adherent cells, cells that stick to the plastic on the bottom of the flask.

Before you get started, you should clean your hood like you learned how to do in your Aseptic Technique class. If you would like to review, watch the video below.

### Learning Objectives:

- 1.1 - Transfer mammalian cells to media.