Lab #1: Thawing of Cells

Thawing your cells should take less than an hour, but you should plan an hour just in case. If you have any questions before getting started you can contact your instructor.

Materials:

- α-MEM medium + 10% FBS +Antibiotics (complete media)
- Frozen cell culture in cryovial.
- T-25 Flask

Procedure:

1. Warm fresh culture media to 37 ºC.
2. Transfer 10 ml of warmed media to a new T-25 flask.
3. Thaw frozen cells quickly at 37°
4. Transfer thawed cells to T-25 flask containing warmed media.
5. Incubate at 37°