This task should take you less than half an hour. However, it’s a good idea to plan on an hour.

Feeding Adherent Cells

Materials:

- Growing cells in T25-flask.
- α-MEM medium (serum-free media)
- α-MEM medium + 10% FBS +Antibiotics (complete media)

Procedure:

1. Warm fresh culture media to 37 ºC.
2. Aspirate culture medium of cells.
3. Wash cells gently by adding 10 ml of α-MEM medium (serum-free media).
4. Remove serum-free culture medium from cells.
5. Add 10 ml. complete media to growing cells in T25-flask.

Remember: The next step is to come into the lab to practice with the materials, view a demonstration, or to even just ask questions. Also, if you are ready to take the lab final, please inform your instructor. The lab final will be performed by yourself and will be graded with a rubric.

Submission

- Image of cells before
- Image of cells after