Introduction to qPCR
Overview of qPCR (Links to an external site.)
Note that the video above refers to Cq values; Cq (Cycle quantitation) is also known as CT (Cycle threshold).
SYBR Green and melt curves
Finding Multiple Melt-Curve Peaks When Using SYBR® Green in Real-Time PCR -- Ask TaqMan®: Ep. 8 (Links to an external site.)
How TaqMan works
How TaqMan Works -- Ask TaqMan® Ep. 13 by Life Technologies (Links to an external site.)
Real-Time PCR Thresholds and Where to Place Them
Real-Time PCR Thresholds and Where to Place Them -- Ask TaqMan®: Ep. 6 (Links to an external site.)
The Purpose of Rox
The Purpose of ROX™ in Real-Time PCR -- Ask TaqMan®: Ep. 7 (Links to an external site.)
Getting Reliable Data in qPCR
Getting Reliable Data in qPCR - Ask TaqMan #22 (Links to an external site.)
Real-Time PCR Applications
Real-time PCR Applications -- Ask TaqMan® Ep. 17 by Life Technologies (Links to an external site.)