Pichia pastoris - HSA Core Production System Laboratory Manual

The Northeast Biomanufacturing Center and Collaborative (NBC2) has created four laboratory manuals provided as downloadable pdfs. The Pichia pastoris - HSA Core Production System laboratory manual is 108 pages; it includes standard operating procedures, batch records, and competencies lists related to upstream processing, downstream processing, and quality control biochemistry.

Below is a list of the files contained within the .zip attachment. The size of each file is included in parenthesis.

Pichia pastoris HSA Core Production (13 files 12.1 MB)

• Pichia pastoris - HSA Core Production System Table of Contents (769435260912792185-pichia-pastoris-restricted.pdf 3.0 MB)
• SOPs
  • Bio-Tek Elx808UI Automated Microplate Reader Standalone SOP (3167636653604819-biotek-elx-8080ui-automated-microplate-reader-sop.pdf 704 KB)
  • New Brunswick BioFlo 3000 Bioreactor SOP (57601621310403145-new-brunswick-bioflo-3000-bioreactor-sop.pdf 1.1 MB)
  • SDS-PAGE SOP (74523064768154312-sds-page-sop.pdf 621 KB)
  • BioLogic LP Chromatography System Operating SOP (151423498748132996-biologic-lp-chromatography-system-operation-sop.pdf 1.2 MB)
  • HSA Production from Pichia pastoris Downstream Process (186652347276509414-hsa-production-from-pichia-pastoris-downstream-processing-batch-record.pdf 641 KB)
  • HSA ELISA SOP (238221252782154934-hsa-elisa-sop.pdf 640 KB)
  • HSA Production from Pichia pastoris Upstream Process (317411851828261004-hsa-production-from-pichia-pastoris-upstream-processing-batch-record.pdf 660 KB)
  • Process Controlled Fed-Batch Fermentation of Recombinant HSA Secreting Pichia pastrois SOP (492891815401175508-process-controlled-fed-batch-fermentation-of-recombinant-hsa-secreting-pichia-pastoris-sop.pdf 683 KB)
  • Xcell SureLock Mini-Cell Gel Box SOP (576312817668496605-xcell-surelock-mini-cell-gel-box-sop.pdf 1 MB)
  • Millipore Pellicon XL Tangential Flow Filter SOP (816283518190127691-millipore-pellicon-xl-tangential-flow-filter-sop.pdf 622 KB)
  • Tangential Flow and Diafiltration of HSA (822059756614823604-tangential-flow-filtration-of-hsa-process-sop.pdf 623 KB)
  • Affinity Chromatography of HSA (854123883949617814-affinity-chromatography-of-hsa-sop.pdf 608 KB)