NISTmAb and NISTCHO: Reference Materials for the Biopharmaceutical Industry

This video, from Digital World Biology, discusses the development and characteristics of a cell line called NIST CHO, engineered to produce a monoclonal antibody. The video details the various cell lines used in research, including E. coli, NS0 cells, and yeast strains, highlighting the transition to CHO cells desired by industry for industrial production. The criteria for the NIST CHO cells are outlined, emphasizing their representation of antibodies produced in CHO cell lines and animal-origin-free production. The development process involves engineering a stable construct with one expression cassette for the antibody's heavy and light chains. Additionally, the availability of NIST CHO cells and their usage terms are discussed, including restrictions on commercial use and the requirement to communicate research results to NIST annually. 

This video runs 43:35 minutes in length.