Comparison of Cryopreservation Conditions on the Performance of NISTCHO Cells

This 10-page article, from the Journal of Advanced Technological Education (J ATE), covers a study that investigates "... the performance of NISTCHO cells following cryopreservation at temperatures of -80C and -150C." The study compared initial cell viability, maximum cell density in culture, and monoclonal antibody production for cells that were cryopreserved for as long as 30 weeks. The cells were thawed and cultured at two-week intervals to observe their growth patterns, peak cell densities, and levels of antibody production. 

This article includes the following sections and subsections: Abstract, Introduction, Methods, Cryptopreservation, NISTCHO Cell Culture, Cell harvest and mAb purification, SDS-PAGE, Results and Discussion, Conclusion, and References.